A Systematic Analysis of Tinman Function Reveals Eya and JAK-STAT Signaling as Essential Regulators of Muscle Development

被引：72

作者：

Liu, Ya-Hsin ^{[1
]}

Jakobsen, Janus S. ^{[1
]}

Valentin, Guillaume ^{[1
]}

Amarantos, Ioannis ^{[1
]}

Gilmour, Darren T. ^{[1
]}

Furlong, Eileen E. M. ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

DEVELOPMENTAL CELL | 2009年 / 16卷 / 02期

关键词：

EARLY MESODERM DEVELOPMENT; DROSOPHILA-MELANOGASTER; JAK/STAT PATHWAY; TRANSCRIPTIONAL NETWORK; TARGET GENES; BOX GENES; KEY ROLE; IN-VIVO; EXPRESSION; ZEBRAFISH;

D O I：

10.1016/j.devcel.2009.01.006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nk-2 proteins are essential developmental regulators from flies to humans. In Drosophila, the family member tinman is the major regulator of cell fate within the dorsal mesoderm, including heart, visceral, and dorsal somatic muscle. To decipher Tinman's direct regulatory role, we performed a time course of ChIP-on-chip experiments, revealing a more prominent role in somatic muscle specification than previously anticipated. Through the combination of transgenic enhancer-reporter assays, colocalization studies, and phenotypic analyses, we uncovered two additional factors within this myogenic network: by activating eyes absent, Tinman's regulatory network extends beyond developmental stages and tissues where it is expressed; by regulating stat92E expression, Tinman modulates the transcriptional readout of JAK/STAT signaling. We show that this pathway is essential for somatic muscle development in Drosophila and for myotome morphogenesis in zebrafish. Taken together, these data uncover a conserved requirement for JAK/STAT signaling and an important component of the transcriptional network driving myogenesis.

引用

页码：280 / 291

页数：12

共 43 条

[1] Functional roles of STAT family proteins: Lessons from knockout mice [J].