A perspective on proteomics in cell biology

被引：50

作者：

Ahmad, Yasmeen ^{[1
]}

Lamond, Angus I. ^{[1
]}

机构：

[1] Univ Dundee, Coll Life Sci, Ctr Gene Regulat & Express, Dundee DD1 5EH, Scotland

来源：

TRENDS IN CELL BIOLOGY | 2014年 / 24卷 / 04期

关键词：

COMPLEX PROTEIN MIXTURES; MASS-SPECTROMETRY; ABSOLUTE QUANTIFICATION; IDENTIFICATION; TANDEM; QUANTITATION; ACCURATE; EXPRESSION; THROUGHPUT; STRATEGY;

D O I：

10.1016/j.tcb.2013.10.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

During the past 15 years mass spectrometry (MS)-based analyses have become established as the method of choice for direct protein identification and measurement. Owing to the remarkable improvements in the sensitivity and resolution of MS instruments, this technology has revolutionised the opportunities available for the system-wide characterisation of proteins, with wide applications across virtually the whole of cell biology. In this article we provide a perspective on the current state of the art and discuss how the future of cell biology research may benefit from further developments and applications in the field of MS and proteomics, highlighting the major challenges ahead for the community in organising the effective sharing and integration of the resulting data mountain.

引用

页码：257 / 264

页数：8

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