Characterization of the tight junction protein ZO-2 localized at the nucleus of epithelial cells

被引：72

作者：

Jaramillo, BE ^{[1
]}

Ponce, A ^{[1
]}

Moreno, J ^{[1
]}

Betanzos, A ^{[1
]}

Huerta, M ^{[1
]}

Lopez-Bayghen, E ^{[1
]}

Gonzalez-Mariscal, L ^{[1
]}

机构：

[1] CINVESTAV, Ctr Adv Res & Adv Studies, Dept Physiol Biophys & Neurosci, Mexico City 07000, DF, Mexico

来源：

EXPERIMENTAL CELL RESEARCH | 2004年 / 297卷 / 01期

关键词：

tight junctions; ZO-2; MAGUK; nuclear matrix; NLS; NES; PKC;

D O I：

10.1016/j.yexcr.2004.03.021

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B-1 and actin from the nuclei of sparse cultures. We show that ZO-2 presents several NLS at its amino region, that when deleted, diminish the nuclear import of the ZO-2 amino segment and impair the ability of the region to regulate the transcriptional activity of promoters controlled by AP-1. Several RS repeats are detected in the ZO-2 amino segment, however, their deletion does not preclude the display of a speckled nuclear pattern. ZO-2 displays two putative NES. However, only the second one appears to be functional, as when conjugated to ovalbumin (OV), it is able to translocate this protein from the nucleus to the cytoplasm in a leptomycin B-sensitive way. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：247 / 258

页数：12

共 45 条

[1] Loss of DNA loop supercoiling and organization in cells infected by herpes simplex virus type 1 [J].