Co-localization of PKCε with various GABAA receptor subunits in the mouse limbic system

被引：14

作者：

Olive, MF ^{[1
]}

Hodge, CW ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Neurol, Ernest Gallo Clin & Res Ctr, Emeryville, CA 94608 USA

来源：

NEUROREPORT | 2000年 / 11卷 / 04期

关键词：

basolateral amygdala; cingulate cortex; dentate gyrus; epsilon; GABA; limbic system; nucleus accumbens; prelimbic cortex; protein kinase C; receptor;

D O I：

10.1097/00001756-200003200-00006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The distribution of PKC epsilon and its co-localization with various GABA(A) receptor subunits within limbic structures of the mouse brain was examined by fluorescence immunohistochemistry. Levels of PKC epsilon immunoreactivity were highest in the cingulate cortex and dentate gyrus, moderate in the nucleus accumbens, and lowest in the prelimbic cortex and basolateral amygdala. Co-localization of PKC epsilon immunoreactivity with the GABA(A) receptor alpha(1), beta(2/3), and gamma(2) subunits varied by subunit and brain region examined, with the majority of co-localization occuring in the dentate gyrus, nucleus accumbens and basolateral amygdala. These results demonstrate that PKC epsilon may interact with GABA(A) receptors in a subunit- and region-specific manner, and provide a potential anatomical basis for recent behavioral and biochemical evidence that PKC epsilon modulates GABA(A) receptor function. NeuroReport 11:683-687 (C) 2000 Lippincott Williams & Wilkins.

引用

页码：683 / 687

页数：5

共 25 条

[1]

Brandon NJ, 1999, J NEUROSCI, V19, P9228

[2] PROTEIN KINASE-C AND CAMP-DEPENDENT PROTEIN-KINASE PHOSPHORYLATE THE BETA-SUBUNIT OF THE PURIFIED GAMMA-AMINOBUTYRIC ACID-A RECEPTOR [J].

BROWNING, MD ;

BUREAU, M ;

DUDEK, EM ;

OLSEN, RW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1315-1318

[3] Brain GABA(A) receptors studied with subunit-specific antibodies [J].

DeBlas, AL .

MOLECULAR NEUROBIOLOGY, 1996, 12 (01) :55-71

[4]

Franklin K B J, 2008, MOUSE BRAIN STEREOTA

[5] MUTANT MICE LACKING THE GAMMA-ISOFORM OF PROTEIN-KINASE-C SHOW DECREASED BEHAVIORAL ACTIONS OF ETHANOL AND ALTERED FUNCTION OF GAMMA-AMINOBUTYRATE TYPE-A RECEPTORS [J].

HARRIS, RA ;

MCQUILKIN, SJ ;

PAYLOR, R ;

ABELIOVICH, A ;

TONEGAWA, S ;

WEHNER, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (09) :3658-3662

[6] Supersensitivity to allosteric GABAA receptor modulators and alcohol in mice lacking PKCε [J].

Hodge, CW ;

Mehmert, KK ;

Kelley, SP ;

McMahon, T ;

Haywood, A ;

Olive, MF ;

Wang, D ;

Sanchez-Perez, AM ;

Messing, RO .

NATURE NEUROSCIENCE, 1999, 2 (11) :997-1002

[7]

HUANG FL, 1987, J BIOL CHEM, V262, P15714

[8]

KELLENBERGER S, 1992, J BIOL CHEM, V267, P25660

[9] A novel nociceptor signaling pathway revealed in protein kinase C ε mutant mice [J].

Khasar, SG ;

Lin, YH ;

Martin, A ;

Dadgar, J ;

McMahon, T ;

Wang, D ;

Hundle, B ;

Aley, KO ;

Isenberg, W ;

McCarter, G ;

Green, PG ;

Hodge, CW ;

Levine, JD ;

Messing, RO .

NEURON, 1999, 24 (01) :253-260

[10] REGULATION OF GABA(A) RECEPTOR FUNCTION BY PROTEIN-KINASE-C PHOSPHORYLATION [J].

KRISHEK, BJ ;

XIE, XM ;

BLACKSTONE, C ;

HUGANIR, RL ;

MOSS, SJ ;

SMART, TG .

NEURON, 1994, 12 (05) :1081-1095

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