Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin

被引：63

作者：

Wentworth, M ^{[1
]}

Ruban, AV ^{[1
]}

Horton, P ^{[1
]}

机构：

[1] Univ Sheffield, Dept Mol Biol & Biotechnol, Robert Hill Inst Photosynth Res, Sheffield S10 2TN, S Yorkshire, England

来源：

FEBS LETTERS | 2000年 / 471卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

non-photochemical quenching; light harvesting complex; xanthophyll cycle; chlorophyll fluorescence; thylakoid membrane;

D O I：

10.1016/S0014-5793(00)01369-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Non-photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xanthophyll cycle. A nem in vitro model for this process has been developed. Purified light harvesting complexes above the detergent critical micelle concentration have a stable high fluorescence yield but a rapidly inducible fluorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was without effect, lutein and antheraxanthin induced a marginal response, whereas the violaxanthin analogue, auroxanthin, induced strong quenching. Quenching was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin-dependent alteration in the chlorophyll environment. (C) 2000 Federation of European Biochemical Societies.

引用

页码：71 / 74

页数：4

共 32 条

[1] CAROTENOID-BINDING PROTEINS OF PHOTOSYSTEM-II [J].