Specific adduction of plant lipid transfer protein by an allene oxide generated by 9-lipoxygenase and allene oxide synthase

被引:39
作者
Bakan, Benedicte
Hamberg, Mats
Perrocheau, Ludivine
Maume, Daniel
Rogniaux, Helene
Tranquet, Olivier
Rondeau, Corinne
Blein, Jean-Pierre
Ponchet, Michel
Marion, Didier
机构
[1] INRA, Unite Rech Biopolymeres Interact Assemblages, F-44316 Nantes 03, France
[2] Ecole Natl Vet, F-44307 Nantes, France
[3] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
[4] Univ Bourgogne, ENESAD, INRA, Unite Phytopharm & Biochim Interact Cellulaires, F-21110 Bretenieres, France
[5] UNSA, CNRS, INRA, Unite Rech Interact Plantes Microorganismes & San, F-06903 Sophia Antipolis, France
关键词
D O I
10.1074/jbc.M608580200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipid transfer proteins (LTPs) are ubiquitous plant lipid-binding proteins that have been associated with multiple developmental and stress responses. Although LTPs typically bind fatty acids and fatty acid derivatives in a non-covalent way, studies on the LTPs of barley seeds have identified an abundantly occurring covalently modified form, LTP1b, the lipid ligand of which has resisted clarification. In the present study, this adduct was identified as the alpha-ketol9-hydroxy-10-oxo-12(Z)-octadecenoic acid. Further studies on the formation of LTP1b demonstrated that the ligand was introduced by nucleophilic attack of the free carboxylate group of the Asp-7 residue of the protein at carbon-9 of the allene oxide fatty acid 9(S), 10-epoxy-10,12(Z)octadecadienoic acid. This reactive oxylipin was produced in barley seeds by oxygenation of linoleic acid by 9-lipoxygenase followed by dehydration of the resulting hydroperoxide by allene oxide synthase. The generation of protein-oxylipin adducts represents a new function for plant allene oxide synthases, enzymes that have earlier been implicated mainly in the biosynthesis of the jasmonate family of plant hormones. Additionally, the LTP-allene oxide synthase interaction opens new perspectives regarding the roles of LTPs in the signaling of plant defense and development.
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页码:38981 / 38988
页数:8
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