High resolution comparative genomic hybridization detects 7-8 megabasepair deletion in PCR amplified DNA

被引：3

作者：

Larsen, J

Ottesen, AM

Kirchhoff, M

Lundsteen, C

Larsen, JK

机构：

[1] Rigshosp, Finsen Ctr, Finsen Lab, DK-2100 Copenhagen, Denmark

[2] Rigshosp, Juliane Marie Ctr, Dept Growth & Reprod, DK-2100 Copenhagen, Denmark

[3] Rigshosp, Juliane Marie Ctr, Dept Clin Genet, DK-2100 Copenhagen, Denmark

来源：

ANALYTICAL CELLULAR PATHOLOGY | 2001年 / 23卷 / 02期

关键词：

high resolution comparative genomic hybridization; DOP-PCR; B-cell leukemia;

D O I：

10.1155/2001/301570

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP-PCR) as opposed to the use of unamplified DNA. Five DNA samples from B-cell leukemias with small 11q deletions were amplified by DOP-PCR and analysed by means of high resolution comparative genomic hybridization (HR-CGH) for the evaluation of aberration size detection limit. By means of HR-CGH, we found the detection limit of DOP-PCR CGH for deletions to be between 3 Mbp and 7-8 Mbp.

引用

页码：61 / 64

页数：4

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