Influence of sample plug width in capillary electrochromatography

被引：28

作者：

Pyell, U

Rebscher, H

Banholczer, A

机构：

[1] Fachbereich Chemie, Philipps-Universität Marburg, D-35032 Marburg, Hans-Meerwein-Strasse

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 779卷 / 1-2期

关键词：

electrochromatography; band broadening; injection parameters; computer simulation; benzoates; polynuclear aromatic hydrocarbons;

D O I：

10.1016/S0021-9673(97)00398-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrochromatography (CEC) is a highly efficient miniaturized chromatographic technique that is very sensitive to extra-column band broadening effects. It is investigated whether band broadening due to the sample injection process is within a tolerable range for the standard sample injection procedures usually employed in CEC. Equations are derived that permit the user of CEC to calculate optimum injection parameters. These equations operate with magnitudes easily accessible in CEC (dimensions of column, plate number and electroosmotic mobility). (C) 1997 Elsevier Science B.V.

引用

页码：155 / 163

页数：9

共 27 条

[1] USE OF LASER DETECTORS IN CAPILLARY LIQUID-CHROMATOGRAPHY [J].