Hepatitis C virus entry depends on clathrin-mediated endocytosis

被引:435
作者
Blanchard, Emmanuelle
Belouzard, Sandrine
Goueslain, Lucie
Wakita, Takaji
Dubuisson, Jean
Wychowski, Czeslaw
Rouille, Yves
机构
[1] Inst Biol Lille, CNRS, UMR 8161, Equipe Hepatite C, F-59021 Lille, France
[2] Inst Pasteur, F-59021 Lille, France
[3] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan
关键词
D O I
10.1128/JVI.00024-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Due to difficulties in cell culture propagation, the mechanisms of hepatitis C virus (HCV) entry are poorly understood. Here, postbinding cellular mechanisms of HCV entry were studied using both retroviral particles pseudotyped with HCV envelope glycoproteins (HCVpp) and the HCV clone JFH-1 propagated in cell culture (HCVcc). HCVpp entry was measured by quantitative real-time PCR after 3 h of contact with target cells, and HCVcc infection was quantified by immunoblot analysis and immunofluorescence detection of HCV proteins expressed in infected cells. The functional role of clathrin-mediated endocytosis in HCV entry was assessed by small interfering RNA-mediated clathrin heavy chain depletion and with chlorpromazine, an inhibitor of clathrin-coated pit formation at the plasma membrane. In both conditions, HCVpp entry and HCVcc infection were inhibited. HCVcc infection was also inhibited by pretreating target cells with bafilomycin A1 or chloroquine, two drugs known to interfere with endosome acidification. These data indicate that HCV enters target cells by clathrin-mediated endocytosis, followed by a fusion step from within an acidic endosomal compartment.
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收藏
页码:6964 / 6972
页数:9
相关论文
共 59 条
[1]   Hepatitis C virus and other Flaviviridae viruses enter cells via low density lipoprotein receptor [J].
Agnello, V ;
Abel, G ;
Elfahal, M ;
Knight, GB ;
Zhang, QX .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12766-12771
[2]   Cellular binding of hepatitis C virus envelope glycoprotein E2 requires cell surface heparan sulfate [J].
Barth, H ;
Schäfer, C ;
Adah, MI ;
Zhang, FM ;
Linhardt, RJ ;
Toyoda, H ;
Kinoshita-Toyoda, A ;
Toida, T ;
van Kuppevelt, TH ;
Depla, E ;
von Weizsäcker, F ;
Blum, HE ;
Baumert, TF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (42) :41003-41012
[3]   Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor [J].
Bartosch, B ;
Vitelli, A ;
Granier, C ;
Goujon, C ;
Dubuisson, J ;
Pascale, S ;
Scarselli, E ;
Cortese, R ;
Nicosia, A ;
Cosset, FL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (43) :41624-41630
[4]   Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes [J].
Bartosch, B ;
Dubuisson, J ;
Cosset, FL .
JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 197 (05) :633-642
[5]   MECHANISM OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS ENTRY INTO CELLS [J].
BORROW, P ;
OLDSTONE, MBA .
VIROLOGY, 1994, 198 (01) :1-9
[6]  
Carbone R, 1997, CANCER RES, V57, P5498
[7]   ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].
CHOO, QL ;
KUO, G ;
WEINER, AJ ;
OVERBY, LR ;
BRADLEY, DW ;
HOUGHTON, M .
SCIENCE, 1989, 244 (4902) :359-362
[8]   Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway [J].
Chu, JJH ;
Ng, ML .
JOURNAL OF VIROLOGY, 2004, 78 (19) :10543-10555
[9]   Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles [J].
Clayton, RF ;
Owsianka, A ;
Aitken, J ;
Graham, S ;
Bhella, D ;
Patel, AH .
JOURNAL OF VIROLOGY, 2002, 76 (15) :7672-7682
[10]   Regulated portals of entry into the cell [J].
Conner, SD ;
Schmid, SL .
NATURE, 2003, 422 (6927) :37-44