Subunit interactions in yeast transcription/repair factor TFIIH - Requirement for Tfb3 subunit in nucleotide excision repair

被引：21

作者：

Feaver, WJ

Huang, WY

Gileadi, O

Myers, L

Gustafsson, CM

Kornberg, RD

Friedberg, EC

机构：

[1] Univ Texas, SW Med Ctr, Dept Pathol, Lab Mol Pathol, Dallas, TX 75235 USA

[2] Weizmann Inst Sci, Dept Mol Genet & Virol, IL-76100 Rehovot, Israel

[3] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 08期

关键词：

D O I：

10.1074/jbc.275.8.5941

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ih yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro, Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb8, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.

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页码：5941 / 5946

页数：6

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