Muscle-based gene therapy and tissue engineering for treatment of growth plate injuries

被引:21
作者
Lee, CW [1 ]
Martinek, V [1 ]
Usas, A [1 ]
Musgrave, D [1 ]
Pickvance, EA [1 ]
Robbins, P [1 ]
Moreland, MS [1 ]
Fu, FH [1 ]
Huard, J [1 ]
机构
[1] Childrens Hosp Pittsburgh, Rangos Res Ctr 4151, Dept Mol Genet & Biochem, Pittsburgh, PA 15213 USA
关键词
BMP-2; gene therapy; IGF-1; muscle; physeal defect; tissue engineering;
D O I
10.1097/00004694-200209000-00001
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Growth plate injuries may lead to a progressive angular deformity or longitudinal growth disturbance. The authors investigated the feasibility of gene therapy and tissue engineering based on autologous muscle- and adenoviral-mediated gene transfer of insulin-like growth factor-1 (IGF-1)and bone morphogenetic protein-2 (BMP-2) to treat tibial physeal defects in rabbits. The medial half of the left proximal tibial growth plate was completely excised in 44 6-week-old New Zealand white rabbits. Four experimental groups were created: no treatment (I), autologous muscle interposition (II), autologous muscle interposition injected with adIGF-1 (III), and autologous muscle interposition injected with adBMP-2 (IV). Radiographic and histologic assessments were obtained postoperatively. Significant tibial shortening and a compact osseous bridge were observed in groups I and IV. Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates.
引用
收藏
页码:565 / 572
页数:8
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