Muscle-based gene therapy and tissue engineering for treatment of growth plate injuries

被引：21

作者：

Lee, CW ^{[1
]}

Martinek, V ^{[1
]}

Usas, A ^{[1
]}

Musgrave, D ^{[1
]}

Pickvance, EA ^{[1
]}

Robbins, P ^{[1
]}

Moreland, MS ^{[1
]}

Fu, FH ^{[1
]}

Huard, J ^{[1
]}

机构：

[1] Childrens Hosp Pittsburgh, Rangos Res Ctr 4151, Dept Mol Genet & Biochem, Pittsburgh, PA 15213 USA

来源：

JOURNAL OF PEDIATRIC ORTHOPAEDICS | 2002年 / 22卷 / 05期

关键词：

BMP-2; gene therapy; IGF-1; muscle; physeal defect; tissue engineering;

D O I：

10.1097/00004694-200209000-00001

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Growth plate injuries may lead to a progressive angular deformity or longitudinal growth disturbance. The authors investigated the feasibility of gene therapy and tissue engineering based on autologous muscle- and adenoviral-mediated gene transfer of insulin-like growth factor-1 (IGF-1)and bone morphogenetic protein-2 (BMP-2) to treat tibial physeal defects in rabbits. The medial half of the left proximal tibial growth plate was completely excised in 44 6-week-old New Zealand white rabbits. Four experimental groups were created: no treatment (I), autologous muscle interposition (II), autologous muscle interposition injected with adIGF-1 (III), and autologous muscle interposition injected with adBMP-2 (IV). Radiographic and histologic assessments were obtained postoperatively. Significant tibial shortening and a compact osseous bridge were observed in groups I and IV. Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates.

引用

页码：565 / 572

页数：8

共 40 条

[1] Biodegradable implants for the treatment of osteochondral defects in a goat model [J].