Comparison of strategies for the construction of libraries of artificial antibodies

被引：10

作者：

Iba, Y ^{[1
]}

Kurosawa, Y ^{[1
]}

机构：

[1] FUJITA HLTH UNIV,SCH MED,INST COMPREHENS MED SCI,TOYOAKE,AICHI 47011,JAPAN

来源：

IMMUNOLOGY AND CELL BIOLOGY | 1997年 / 75卷 / 02期

关键词：

antibody repertoire; antigen-antibody interactions; artificial antibody; expression vectors; phage-display systems; selection systems;

D O I：

10.1038/icb.1997.33

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Construction of libraries of artificial antibodies has been reported by several groups of investigators. Various forms of antibody fused to a surface protein, cpIII, are expressed on the surface of filamentous phage. Since phages that encode desired antibodies can be easily grown in Escherichia coli after selection with target antigens, the phage-display antibody system appears to be very useful for various biological purposes. In this brief review, recent progress in research into the production of artificial antibodies is summarized and the strategies used for the construction of libraries of artificial antibodies are compared.

引用

页码：217 / 221

页数：5

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