Derivatization of small biomolecules for optimized matrix-assisted laser desorption/ionization mass spectrometry

被引：52

作者：

Tholey, A ^{[1
]}

Wittmann, C ^{[1
]}

Kang, MJ ^{[1
]}

Bungert, D ^{[1
]}

Hollemeyer, K ^{[1
]}

Heinzle, E ^{[1
]}

机构：

[1] Univ Saarland, Tech Biochem, D-66123 Saarbrucken, Germany

来源：

JOURNAL OF MASS SPECTROMETRY | 2002年 / 37卷 / 09期

关键词：

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry; metabolism; low molecular mass compounds; derivatization; bioanalysis;

D O I：

10.1002/jms.355

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is a powerful tool for the measurement of low molecular mass compounds of biological interest. The limitations for this method are the volatility of many analytes, possible interference with matrix signals or bad ionization or desorption behavior of the compounds. We investigated the application of well-known and straightforward one-pot derivatization procedures to circumvent these problems. The derivatizations tested allow the measurement and the labeling of alcohols, aldehydes and ketones, carboxylic acids, alpha-ketocarboxylic acids and amines. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：963 / 973

页数：11

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