Measuring diffusion in cell membranes by fluorescence correlation spectroscopy

被引：80

作者：

Sengupta, P ^{[1
]}

Balaji, J ^{[1
]}

Maiti, S ^{[1
]}

机构：

[1] Tata Inst Fundamental Res, Dept Chem Sci, Colaba 400005, Mumbai, India

来源：

METHODS | 2002年 / 27卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S1046-2023(02)00096-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence Correlation Spectroscopy (FCS) can measure diffusion on the cell surface with unparalleled sensitivity. In appropriate situations, this can be the most sensitive and accurate method for measuring receptor interaction and oligomerization. Here we attempt to describe FCS in sufficient detail so that the reader is able to judge when there is a compelling reason to choose this technique, understand the basic theory behind it, construct a FCS spectrometer in the laboratory, and analyze the data to obtain a meaningful estimate of the physical parameters. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：374 / 387

页数：14

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