The thermodynamic basis of mechanisms of bacterial virus infection

被引:6
作者
Mrevlishvili, GM
Mdzinarashvili, T
Al-Zaza, M
Tsinadze, L
Tushishvili, D
Razmadze, G
机构
[1] Tbilisi State Univ, Dept Phys, GE-380028 Tbilisi, Georgia
[2] Georgian Acad Sci, Inst Bacteriophage Microbiol & Virol, GE-380001 Tbilisi, Georgia
关键词
D O I
10.1351/pac199971071291
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
By using of differential scanning calorimetry (DSC) and temperature scanning rotational viscosimetry the specific phase transition we study in the heated suspensions of phages (Sd, DDVI) modeling the process of injection of the genetic material from the phage: the transition of interphage ds-DNA from the state of intramolecular liquid crystal into the coiled state of ds-DNA. The other conformational rearrangements represent the thermal denaturation of the coat proteins and helix-coil transition in DNA. We detect that: 1 ds-DNA output from the phage head (capsid), induced by temperature, not accompanied by any thermal (endo-or exo-) heat effects (temperature interval 48-60 degrees C; DSC sensitivity 0.4 mu W/cm). 2 ds-DNA output from the capsid, increase of the suspension viscosity at least 100-times (!) and recent experiments reveal that it is not one-step process; we have at least two distinct phases of outlet of genetic material, manifesting, that there are strong interactions between the DNA sites and some proteins inside the capsid. It is not excluded that kinetic factors also regulate this ejection process. 3 According to our working hypothesis the main factors of the DNA condensation and packaging in viruse head and after its ejection through the hole with diameter close to dsDNA's, are caused by the surrounded solution 'quality' and so-called 'hydration forces' between ds-DNA parallel packaged segments and, more exactly, by the difference of this parameters inside and outside the capsid of the phage.
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页码:1291 / 1299
页数:9
相关论文
共 27 条
  • [1] STUDIES OF VIRUS STRUCTURE BY LASER RAMAN-SPECTROSCOPY .38. SECONDARY STRUCTURE AND INTERACTIONS OF THE PACKAGED DSDNA GENOME OF BACTERIOPHAGE-P22 INVESTIGATED BY RAMAN DIFFERENCE SPECTROSCOPY
    AUBREY, KL
    CASJENS, SR
    THOMAS, GJ
    [J]. BIOCHEMISTRY, 1992, 31 (47) : 11835 - 11842
  • [2] Black Lindsay W., 1994, P218
  • [3] ION ETCHING OF BACTERIOPHAGE-T4 - SUPPORT FOR A SPIRAL-FOLD MODEL OF PACKAGED DNA
    BLACK, LW
    NEWCOMB, WW
    BORING, JW
    BROWN, JC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (23) : 7960 - 7964
  • [4] DEMBO A. T., 1965, BIOFEZIKA, V10, P404
  • [5] DEMBO AT, 1983, KRISTALLOGRAFIYA+, V28, P1105
  • [6] DOBROV EN, 1976, VOP MED KHIM, V22, P98
  • [7] DNA ARRANGEMENT IN ISOMETRIC PHAGE HEADS
    EARNSHAW, WC
    HARRISON, SC
    [J]. NATURE, 1977, 268 (5621) : 598 - 602
  • [8] VISUALIZATION OF THE DNA THREAD PACKING WITHIN BACTERIOPHAGE-T4 HEADS
    FUJIYOSHI, Y
    YAMAGISHI, H
    KUNISADA, T
    SUGISAKI, H
    KOBAYASHI, T
    UYEDA, N
    [J]. JOURNAL OF ULTRASTRUCTURE RESEARCH, 1982, 79 (02): : 235 - 240
  • [9] GABASHVILI I, 1993, J BIOMOLECULAR STRUC, V5, P705
  • [10] GABASHVILI IS, 1991, BIOFIZIKA+, V36, P788