Recovery of soluble, active recombinant protein from inclusion bodies

被引：40

作者：

Shi, PY ^{[1
]}

Maizels, N ^{[1
]}

Weiner, AM ^{[1
]}

机构：

[1] YALE UNIV,DEPT BIOCHEM & MOL BIOPHYS,SCH MED,NEW HAVEN,CT 06520

来源：

BIOTECHNIQUES | 1997年 / 23卷 / 06期

关键词：

D O I：

10.2144/97236bm15

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：1036 / 1038

页数：3

共 11 条

[1]

BUNCHNER J, 1991, BIOCHEMISTRY-US, V30, P1586

[2]

CREIGHTON T. E., 1985, UCLA S MOL CELLULAR, V39, P249

[3]

CUDNY H, 1986, J BIOL CHEM, V261, P6450

[4]

Deutscher M. P., 1982, Enzymes, V15, P183, DOI [10.1016/S1874-6047(08)60279-6, DOI 10.1016/S1874-6047(08)60279-6]

[5] HIGH-PERFORMANCE HYDROPHOBIC INTERACTION CHROMATOGRAPHY AS A TOOL FOR PROTEIN REFOLDING [J].

GENG, XD ;

CHANG, XQ .

JOURNAL OF CHROMATOGRAPHY, 1992, 599 (1-2) :185-194

[6] CYANATE FORMATION IN SOLUTIONS OF UREA .1. CALCULATION OF CYANATE CONCENTRATIONS AT DIFFERENT TEMPERATURE AND PH [J].

HAGEL, P ;

GERDING, JJT ;

FIEGGEN, W ;

BLOEMENDAL, H .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 243 (03) :366-+

[7] Thermolabile folding intermediates: Inclusion body precursors and chaperonin substrates [J].

King, J ;

HaasePettingell, C ;

Robinson, AS ;

Speed, M ;

Mitraki, A .

FASEB JOURNAL, 1996, 10 (01) :57-66

[8]

SINHA D, 1994, BIOTECHNIQUES, V17, P509

[9] Improved refolding of an immobilized fusion protein [J].

Stempfer, G ;

HollNeugebauer, B ;

Rudolph, R .

NATURE BIOTECHNOLOGY, 1996, 14 (03) :329-334

[10] DYNAMICS OF THE CHAPERONIN ATPASE CYCLE - IMPLICATIONS FOR FACILITATED PROTEIN-FOLDING [J].

TODD, MJ ;

VIITANEN, PV ;

LORIMER, GH .

SCIENCE, 1994, 265 (5172) :659-666

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