Polymerase chain reaction typing of nonviable Mycobacterium tuberculosis isolates

被引：3

作者：

Loeffelholz, MJ ^{[1
]}

Thompson, CJ ^{[1
]}

Gaunt, DD ^{[1
]}

Koontz, FP ^{[1
]}

Gilchrist, MJR ^{[1
]}

机构：

[1] UNIV IOWA,COLL MED,DEPT PATHOL,IOWA CITY,IA 52242

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 1996年 / 26卷 / 3-4期

关键词：

D O I：

10.1016/S0732-8893(96)00214-3

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacterium tuberculosis isolates for which both viable and nonviable cultures were available. DNA extracted from the nonviable cultures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the polymorphic GC-rich repetitive sequence. For both isolates, the nonviable cultures generated PCR-banding patterns identical to those generated by the fresh subcultures. (C) 1997 Elsevier Science Inc.

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页码：149 / 151

页数：3

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