A novel and universal method for microRNA RT-qPCR data normalization

被引：817

作者：

Mestdagh, Pieter ^{[1
]}

Van Vlierberghe, Pieter ^{[1
]}

De Weer, An ^{[1
]}

Muth, Daniel ^{[2
]}

Westermann, Frank ^{[2
]}

Speleman, Frank ^{[1
]}

Vandesompele, Jo ^{[1
]}

机构：

[1] Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, Belgium

[2] German Canc Res Ctr, Dept Tumour Genet, D-6900 Heidelberg, Germany

来源：

GENOME BIOLOGY | 2009年 / 10卷 / 06期

关键词：

GENE-EXPRESSION; REFERENCE RNA; PCR; PATTERNS; DIFFERENTIATION; QUANTIFICATION; NEUROBLASTOMA; POLYCISTRON; GUIDELINES; SYSTEM;

D O I：

10.1186/gb-2009-10-6-r64

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR data and compare its performance to the currently adopted approach. We demonstrate that the mean expression value outperforms the current normalization strategy in terms of better reduction of technical variation and more accurate appreciation of biological changes.

引用

页数：10

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