Determination of recombinant protein in animal cell culture supernatant by near-infrared spectroscopy

被引：28

作者：

Harthun, S ^{[1
]}

Matischak, K ^{[1
]}

Friedl, P ^{[1
]}

机构：

[1] TH DARMSTADT,INST BIOCHEM,D-64287 DARMSTADT,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 251卷 / 01期

关键词：

D O I：

10.1006/abio.1997.2218

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the measurement of recombinant protein in animal cell culture supernatants with near-infrared (NIR) absorbance spectroscopy. Human antithrombin III is determined in CHO (Chinese hamster ovary)-T1 medium, both fresh and taken from CHO cell culture. NIR spectra are collected over the frequency range of 1000-2500 nm. A multivariate calibration model is generated by correlating spectral features with concentration values using partial least-squares regression. Separate test sets are used to evaluate the computed model. Protein measurement is established over the range 0.1-5 mu g/mL with a standard error of prediction of less than 0.5 mu g/mL. This result underlines the analytical applicability of NIR spectroscopy for animal cell culture process monitoring. (C) 1997 Academic Press.

引用

页码：73 / 78

页数：6

共 14 条

[1] Simultaneous measurements of glucose, glutamine, ammonia, lactate, and glutamate in aqueous solutions by near-infrared spectroscopy [J].