RNA polymerase III interferes with Ty3 integration

被引：23

作者：

Connolly, CM ^{[1
]}

Sandmeyer, SB ^{[1
]}

机构：

[1] UNIV CALIF IRVINE,COLL MED,DEPT MICROBIOL & MOL GENET,IRVINE,CA 92717

来源：

FEBS LETTERS | 1997年 / 405卷 / 03期

关键词：

integration; retrovirus; RNA polymerase III; transposable element; tRNA gene; Ty3;

D O I：

10.1016/S0014-5793(97)00200-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ty3, a gypsylike retrotransposon of budding yeast, integrates at the transcription initiation site of genes transcribed by RNA polymerase III (pol LII). It was previously shown that integration in vitro requires intact promoter elements and the pol III transcription factors TFIIIB and TFIIIC. In order to test the effect of pol III on integration, increasing amounts of a pol III-containing fraction were added to Ty3 in vitro integration reactions. The pol III-containing fraction was inhibitory to integration. These results are consistent with a model where the Ty3 integration complex and pol III recognize similar features of the stable transcription complex and compete with each other for access to the transcription initiation site. (C) 1997 Federation of European Biochemical Societies.

引用

页码：305 / 311

页数：7

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