IL-1β in irrigation fluid and mRNA expression in synovial tissue of the knee joint as therapeutic markers of inflammation in collagen antibody-induced arthritis

被引：6

作者：

Chia, Wei-Tso ^{[1
,2
]}

Yeh, Li-Tzu ^{[1
]}

Chen, Yuan-Wu ^{[1
]}

Lee, Herng-Sheng ^{[1
]}

Chang, Deh-Ming ^{[1
]}

Sytwu, Huey-Kang ^{[1
]}

机构：

[1] Natl Def Med Ctr, Grad Inst Med Sci, Taipei 114, Taiwan

[2] Hsin Chu Gen Hosp, Dept Hlth, Executive Yuan, Taiwan

来源：

DISEASE MARKERS | 2009年 / 26卷 / 01期

关键词：

FIBROBLAST-LIKE SYNOVIOCYTES; RHEUMATOID-ARTHRITIS; METHOTREXATE; AUTOIMMUNITY; CELLS;

D O I：

10.3233/DMA-2009-0593

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Objective: We studied the relationship between the severity of inflammation and IL-1 beta production and relative expression level of IL-1 beta mRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of a murine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markers for treating rheumatoid arthritis. Methods: Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIA induction. The IL-1 beta protein level was measured by enzyme-linked immunosorbent assay, and the relative expression level of IL-1 beta mRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levels and histopathological changes. Results: IL-1 beta concentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. The relative expression level of IL-1 beta mRNA was elevated in synovial tissue. Histopathological changes in the knee joint and foot indicated similar severity. Conclusions: IL-1 beta concentration in irrigation fluid and relative expression level of IL-1 beta mRNA in the synovium have potential as therapeutic markers in studying and treating CAIA.

引用

页码：1 / 7

页数：7

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