The presence of an extractable substance in the CSF of humans with cerebral vasospasm after subarachnoid haemorrhage that correlates with phosphatase inhibition

The cellular events leading to cerebral vasospasm after subarachnoid haemorrhage are poorly understood, although an increase in smooth muscle myosin light chain phosphorylation has been observed. This study set out to determine if phosphatase inhibition may be involved in the pathological maintenance of tension observed during vasospasm. We found that 1 nM okadaic acid, a type 2A protein phosphatase inhibitor, elicited an increase in rate of O-2 consumption in the porcine carotid artery similar to that by cerebrospinal fluid (CSF) from vasospastic patients (CSFv, it = 5) (control 0.23 +/- 0.03, CSFv 0.84 +/- 0.16 and okadaic acid 0.85 +/- 0.02 mu mol min(-1) g dwt(-1)). It was also observed that phosphatase inhibition with 1 nM okadaic acid significantly slowed relaxation after a stretch in a similar fashion to CSFv haemorrhage. CSF from vasospastic subarachnoid haemorrhage patients, but not from those without vasospasm, contains an extractable substance which modulates myosin light chain phosphorylation in vitro. A phosphatase preparation obtained from the porcine carotid artery dephosphorylated 63 +/- 2% of the phosphorylated (MLC20) substrate in vitro, and non-vasospastic CSF treated enzyme dephosphorylated 60 +/- 2.6%. Okadaic acid inhibited phosphatase dephosphorylated only 7.5 +/- 1% of the substrate where CSFv treated enzyme dephosphorylated 22 +/- 2.8% of the substrate. We conclude that inhibition of smooth muscle phosphatase may be involved in the mechanisms associated with cerebral vasospasm after subarachnoid haemorrhage. (C) 2000 Elsevier Science B.V. All rights reserved.