Cholinesterase of rats experimentally infected by Cryptococcus neoformans: Relationship between inflammatory response and pathological findings

The aim of this study was to assess the role of the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) as biomarkers of inflammation and tissue injury on rats experimentally infected by Cry ptococcus neoformans. For this purpose, 20 male rats were divided into two groups: 10 animals representing the uninfected control group (Group A) and 10 C neoformans var. grubii infected animals (Group B). Blood and brain samples were collected on days 10 (A(10) and B-10), and 30 (A(30) and B-30) post-infection (P1) for hematological analyses; AChE (in lymphocytes and brain) and seric BChE activity; interleukins (IL-I, IL-6, and IL-I 0); nitrite/nitrate (NOx) levels; and markers of protein oxidation (AOPP) and lipid peroxidation (TBARS). As a result, when animals of Group A were compared to animals of Group B, it was observed leukocytosis (P< 0.05) on day 10 P1; AChE activity increase (P< 0.05) in lymphocytes (day 30 P1) and in brain (days 10 and 30 P1); BChE activity decrease (P< 0.05) on day 10 P1; IL-I and IL-6 increase (P< 0.01) in both periods, while IL-b had reduced levels (P< 0.01) in the same periods; NO5 levels increased (P< 0.05) significantly on days 10 and 30 P1, while AOPP and TBARS levels increased significantly on day 30 P1; as well as pneumonia on infected rats. Therefore, based on the results obtained, it was possible to conclude that AChE and BChE behavior lead to a proinflammatory reaction evidenced by the enhancement of IL-I, IL-B, and NO5 throughout the experiment associated with reduction on IL-I 0 levels, and cellular damage. (C) 2015 Elsevier GmbH. All rights reserved.