Degradation of human Aurora-A protein kinase is mediated by hCdh1

被引：94

作者：

Taguchi, S

Honda, K

Sugiura, K

Yamaguchi, A

Furukawa, K

Urano, T

机构：

[1] Nagoya Univ, Sch Med, Dept Biochem 2, Showa Ku, Nagoya, Aichi 4660065, Japan

[2] Fukui Med Univ, Dept Surg 1, Fukui 91011, Japan

来源：

FEBS LETTERS | 2002年 / 519卷 / 1-3期

关键词：

Aurora-A; Cdh1; Cdc20; KEN box; anaphase promoting complex/cyclosome;

D O I：

10.1016/S0014-5793(02)02711-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)-ubiquitin-proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows teat Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：59 / 65

页数：7

共 38 条

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