X-ray microscopy of living multicellular organisms with the Prague Asterix Iodine Laser System

被引：12

作者：

Desai, T

Batani, D

Bernardinello, A

Poletti, G

Orsini, F

Ullschmied, J

Juha, L

Skala, J

Kralikova, B

Krousky, E

Pfeifer, M

Kadlec, C

Mocek, T

Präg, A

Renner, O

Cotelli, F

Lamia, CL

Zullini, A

机构：

[1] Univ Milano Bicocca, Dipartimento Fis G Occhialini, I-20126 Milan, Italy

[2] Univ Milano Bicocca, INFM, I-20126 Milan, Italy

[3] Univ Milano Bicocca, Inst Fisiol Gen & Chim Biol, I-20126 Milan, Italy

[4] Prague Asterix Iodine Laser Syst Res Ctr, Prague, Czech Republic

[5] Univ Milano Bicocca, Dipartimento Biol, I-20126 Milan, Italy

[6] Univ Milano Bicocca, Dipartimento Biotecnol & Biosci, I-20126 Milan, Italy

来源：

LASER AND PARTICLE BEAMS | 2003年 / 21卷 / 04期

关键词：

atomic force microscopy; laser-produced plasmas; multicellular Caenorhabditis elegans; soft X-ray contact microscopy;

D O I：

10.1017/S0263034603214051

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were lambda = 1.314 mum and tau (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I greater than or equal to 10(14) W/cm(2). Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.

引用

页码：511 / 516

页数：6

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