Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry

被引：37

作者：

Kumaratilake, LM ^{[1
]}

Ferrante, A

机构：

[1] Womens & Childrens Hosp, Dept Immunopathol, Adelaide, SA 5006, Australia

[2] Univ Adelaide, Womens & Childrens Hosp, Dept Paediat, Adelaide, SA 5006, Australia

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2000年 / 7卷 / 01期

关键词：

D O I：

10.1128/CDLI.7.1.9-13.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software, To differentiate the proportion of neutrophil surface-bound merozoites from the merozoites ingested by neutrophils, the fluorescence of bound merozoites was quenched by adding trypan blue, The data showed that sera from malaria-immune individuals in the Solomon Islands and Papua New Guinea promoted merozoite engulfment by neutrophils. The cytokines tumor necrosis factor alpha, gamma interferon, granulocyte-macrophage colony-stimulating factor, and interleukin-1 beta significantly increased the amount and the rate of merozoite phagocytosis by neutrophils. Optimum merozoite phagocytosis occurred when both cytokines and anti-malarial antibody were present.

引用

页码：9 / 13

页数：5

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