Both Stat5a and Stat5b are required for antigen-induced eosinophil and T-cell recruitment into the tissue

被引:71
作者
Kagami, S
Nakajima, H
Kumano, K
Suzuki, K
Suto, A
Imada, K
Davey, HW
Saito, Y
Takatsu, K
Leonard, WJ
Iwamoto, I
机构
[1] Chiba Univ, Sch Med, Dept Internal Med 2, Chiba 2608670, Japan
[2] NHLBI, Lab Mol Immunol, Bethesda, MD 20892 USA
[3] AgResearch, Hamilton, New Zealand
[4] Univ Tokyo, Inst Med Sci, Dept Immunol, Tokyo, Japan
关键词
D O I
10.1182/blood.V95.4.1370.004k29_1370_1377
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Antigen-induced eosinophil recruitment into the airways of sensitized mice is mediated by CD4(+) T cells and their cytokines, especially IL-5. In this study, we found that the antigen-induced airway eosinophilia was diminished in Stat5a-deficient (Stat5a(-/-)) mice and Stat5b-deficient (Stat5b(-/-)) mice. We also found that antigen-induced CD4(+) T-cell infiltration and IL-5 production in the airways were diminished in Stat5a(-/-) mice and Stat5b(-/-) mice. Moreover, antigen-induced proliferation of splenocytes was diminished in Stat5a(-/-) mice and Stat5b(-/-) mice, suggesting that the generation of antigen-primed T cells may be compromised in Stat5a(-/-) mice and Stat5b(-/-) mice and this defect may account for the diminished antigen induced T-cell infiltration into the airways. Interestingly, IL-4 and IL-5 production from anti-CD3-stimulated splenocytes was diminished in Stat5a(-/-) mice and Stat5b(-/-) mice. However, antigen-specific IgE and IgG1 production was diminished in Stat5a(-/-) mice but not in Stat5b(-/-) mice, whereas antigen-specific IgG2a production was increased in Stat5a(-/-) mice, suggesting the enhanced Th1 responses in Stat5a(-/-) mice. Finally, we found that eosinophilo-poiesis induced by the administration of recombinant IL-5 was also diminished in Stat5a(-/-) mice and Stat5b(-/-) mice. Together, these results indicate that both Stat5a and Stat5b are essential for induction of antigen-induced eosinophil recruitment into the airways and that the defects in antigen-induced eosinophil recruitment in Stat5a(-/-) mice and Stat5b(-/-) mice result from both impaired IL-5 production in the airways and diminished IL-5 responsiveness of eosinophils. (C) 2000 by The American Society of Hematology.
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页码:1370 / 1377
页数:8
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