Adsorption, sequestration, and bioaccessibility of As(V) in soils

The influence of various soil physical and chemical properties <Fe and Mn oxides, pH, cation exchange capacity, total inorganic and organic carbon, and particle size) on As<V> adsorption, sequestration, and relative bioaccessibility <as a surrogate for oral bioavailability> was investigated in a wide range of well-characterized soils over a 6-month period. Arsenic<V> bioaccessibility was measured using a streamlined version of a physiologically based extraction test <PBET>, designed to replicate the solubility-limiting conditions in a child's digestive tract. The soil's dithionite-citrate-bicarbonate <DCB> extractable Fe oxide content was the most important <and only statistically significant> soil property controlling the initial degree of adsorption. Sequestration, as measured by the reduction in bioaccessibility over time, occurred to a significant extent in 17 of 36 <47.2%> soils over the first 3 months. In contrast, only 4 of 36 <11.1%> soils exhibited a significant reduction in bioaccessibility from 3 to 6 months. Soil pH was the most important <and only statistically significant> soil property affecting the decrease in bioaccessibility upon aging for 6 months. Soils with pH < 6 generally sequestered As<V> more strongly over time, whereas those with pH > 6 generally did not. The Fe oxide content and pH were the most important soil properties governing the steady-state bioaccessibility of As<V> in soil. Two multivariable linear regression models of steady-state As<V> bioaccessibility were developed using soil properties as independent variables. Generally, soils having higher Fe oxide content and lower soil pH exhibited lower bioaccessibility. These models were able to account for similar to75-80% of the variability in steady-state bioaccessibility and independently predict bioaccessibility in five soils within a root-mean-square error <RMSE> of 8.2-10.9%. One of these models was also able to predict within an RMSE of 9.5% the in vivo bioavailability of As in nine contaminated soils previously used in swine dosing trials. These results indicate the bioaccessibility, and thus, potentially the bioavailability of otherwise soluble As<V> added to soils <i.e., the worst-case bioavailability scenario> is significantly reduced in some soils over time, particularly those with lower pH and higher Fe oxide content. These results also provide a means of estimating As<V> bioaccessibility and bioavailability on the basis of soil properties.