This study compared the response of harvests taken in May, June, August and September-October in terms of crop responses (yield, N uptake, and concentrations of crude protein and nitrate) to N fertilizer and residual soil nitrate and ammonium. Three trials were conducted in south coastal British Columbia in 1990-1992 to evaluate the response of an established sward of orchardgrass (Dactylis glomerata L.) to a range of N fertilizer rates. Both yields and daily crop growth rates were highest in cut 1, lowest in cut 4 and intermediate in cuts 2 and 3. For all four cuts, 95 and 90% of maximum yield was attained at about 136 and 82 kg ha(-1) of applied N, respectively. Crop N supply from non-fertilizer sources ranged from 36 to 90 kg N ha(-1), of which about 52% was attributed to nitrate present in the soil prior to growth and about 48% was N released from the soil, translocated from roots or deposited from the atmosphere. At 95% of maximum yield, crude protein concentrations ranged from 147 g kg(-1) in the higher yielding cut 1 to 189 g kg-1 in cuts 2 and 4, while at 90% of maximum yield concentrations were 10 g kg(-1) lower in each cut. Plant nitrate concentrations were close to levels that are toxic to cattle for the 95% target yield, but relatively safe at the 90% yield. The crop removed about 50 kg ha-1 more N when fertilized for 95% of maximum yield than for 90%, which translates to over 300 kg ha(-1) more crude protein. High relative yield leaves behind more soil nitrate after harvest. The results suggest that the first cut should be managed for 95% of maximum yield with about 130 kg N ha(-1.) Cuts 2 and 3 should be managed for 90% of maximum yield, to avoid high plant nitrate concentrations, with 100-110 kg N ha(-1). Cut 4 should be given no more than 50 kg N ha(-1) for less than 90% of maximum yield because of the risk of residual soil nitrates. This study shows for the first time the benefits and disadvantages of applying N at different rates for each harvest over the growing season.