Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia

被引：128

作者：

Pevenstein, SR

Williams, RK

McChesney, D

Mont, EK

Smialek, JE

Straus, SE

机构：

[1] NIAID, Clin Invest Lab, Med Virol Sect, NIH, Bethesda, MD 20892 USA

[2] NCI, Pathol Lab, Serv Anat Pathol, NIH, Bethesda, MD 20892 USA

[3] Univ Maryland, Sch Med, Dept Pathol, Baltimore, MD 21201 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 12期

关键词：

D O I：

10.1128/JVI.73.12.10514-10518.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using rear-time fluorescence PCR, we quantitated the numbers of copies of latent varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) genomes in 15 human trigeminal ganglia. Eight (538) and 1 (7%) of 15 ganglia were PCR positive for HSV-1 or -2 glycoprotein G genes, with means of 2,902 +/- 1,082 (standard error of the mean) or 109 genomes/10(5) cells, respectively. Eleven of 14 (79%) to 13 of 15 (87%) of the ganglia were PCR positive for VZV gene 29, 31, or 62. Pooling of the results for the three VZV genes yielded a mean of 258 +/- 38 genomes/10(5) ganglion cells. These levels of latent viral genome loads have implications for virus distribution in and reactivation from human sensory ganglia.

引用

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页码：10514 / 10518

页数：5

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