Extreme Clonality in Lymphoblastoid Cell Lines with Implications for Allele Specific Expression Analyses

被引:45
作者
Plagnol, Vincent [1 ]
Uz, Elif [2 ]
Wallace, Chris [3 ]
Stevens, Helen [1 ]
Clayton, David [1 ]
Ozcelik, Tayfun [4 ]
Todd, John A. [1 ]
机构
[1] Univ Cambridge, JDRF WT Diabet & Inflammat Lab, Cambridge, England
[2] Bilkent Univ, Fac Sci, Dept Mol Biol & Genet, Ankara, Turkey
[3] William Harvey Res Inst, Barts & The London, London, England
[4] Bilkent Univ, Inst Mat Sci & Nanotechnol UNAM, Ankara, Turkey
来源
PLOS ONE | 2008年 / 3卷 / 08期
基金
英国惠康基金;
关键词
D O I
10.1371/journal.pone.0002966
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays.
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页数:6
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