Gas chromatographic-mass spectrometric determination of natural profiles of androgens, progestogens, and glucocorticoids in muscle tissue of male cattle

被引:19
作者
Fritsche, S [1 ]
Schmidt, G [1 ]
Steinhart, H [1 ]
机构
[1] Univ Hamburg, Inst Biochem & Food Chem, D-20146 Hamburg, Germany
关键词
hormone pattern; gas chromatography-mass spectrometry; beef; bulls; steers;
D O I
10.1007/s002170050515
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A rapid and economical method was developed for the simultaneous determination of androgens, progestogens, and glucocorticoids and their precursors and metabolites in meat. Steroids were isolated with liquid extraction followed by C-18-solid-phase extraction (C-18-SPE). Corticoids were separated from the less polar steroids by Si-SPE. The non-polar steroids required additional purification by filtration through an NH2 cartridge. The fractions were combined and derivatized with a universally applicable trimethylsilylation of both hydroxyl and keto groups. Determination was performed with gas chromatography-mass selective detection (determination limits: 0.02-0.1 mu g/kg). Hormone profiles of 15 muscle tissue samples of both bulls and steers were analysed. Beef from bulls contained higher concentrations of testosterone and epitestosterone (P<0.001), 11-deoxycorticosterone (P<0.01), androstenedione (P<0.05) and cortisone (P<0.05), and significantly lower proportions of cortisol (P<0.001) than beef from steers.
引用
收藏
页码:393 / 399
页数:7
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