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Automated purification of microcystins

被引：15
作者：
Edwards, C
Lawton, LA
Coyle, SM
Ross, P
机构：
[1] ROBERT GORDON UNIV,SCH APPL SCI,ABERDEEN AB1 1HG,SCOTLAND
[2] SHANDON HPLC,RUNCORN WA7 1PR,CHESHIRE,ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 734卷 / 01期
关键词：
preparative chromatography; flash chromatography; sample preparation; microcystins; toxins;
D O I：
10.1016/0021-9673(95)01148-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simplified method for the concentration and purification of microcystins was developed. A 3-I volume of cyanobacterial scum (equivalent to 124 g dry mass of cells) collected from Rutland Water, Leicestershire, in 1989 was extracted in methanol and centrifuged and the supernatant was retained. This was diluted to ca. 20% aqueous methanol and applied to a reversed-phase flash chromatography cartridge. Microcystins were eluted with 70% aqueous methanol then reduced in vacuo. Gradient separation of the extract was optimized on an analytical column and scaled up to a 15 cm X 7.5 cm I.D. column with subsequent purification of individual compounds.
引用
收藏
页码：175 / 182
页数：8
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