Involvement of protein kinase c in responses of rat dorsal horn neurons to mechanical stimuli and periaqueductal gray descending inhibition

被引:27
作者
Peng, YB
Lin, Q
Willis, WD
机构
[1] UNIV TEXAS, MED BRANCH, DEPT ANAT & NEUROSCI, GALVESTON, TX 77555 USA
[2] UNIV TEXAS, MED BRANCH, INST MARINE BIOMED, GALVESTON, TX 77555 USA
关键词
spinal cord; dorsal horn neuron; protein kinase C; pain modulation; periaqueductal gray; rat;
D O I
10.1007/PL00005664
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The effects of a protein kinase C (PKC) activator, 12-O-tetradecanoylphorbol-13-acetate (TPA), on the activity and periaqueductal gray (PAG)-induced inhibition of rat dorsal horn neurons of the lumbar spinal cord were tested. A microdialysis fiber was placed through the dorsal horn for the purpose of local application of pharmacological agents. Extracellular single-unit recordings from dorsal horn neurons were made near the microdialysis fiber. TPA was tested on nociceptive dorsal horn cells. There was a significant increase in the back ground activity and responses to ''brush'', with no changes in responses to pressure and pinch stimuli. TPA also significantly blocked the PAG-induced inhibition of responses to brush, press, and pinch. These effects were eliminated by coadministration of the PKC inhibitor NPC-15437. The solvent, which contained dimethyl sulfoxide, was also tested for its effect on the responses to peripheral mechanical stimuli and FAG-induced inhibition of the dorsal horn neurons. There were no significant changes. This experiment suggests that activation of the PKC second messenger system might increase the activity of dorsal horn neurons and their responses to peripheral stimuli; in addition, the phorbol ester attenuated the FAG-induced descending inhibition of the dorsal horn neuron activity.
引用
收藏
页码:561 / 570
页数:10
相关论文
共 123 条
[1]   STIMULATION-PRODUCED DESCENDING INHIBITION FROM THE PERIAQUEDUCTAL GRAY AND NUCLEUS RAPHE MAGNUS IN THE RAT - MEDIATION BY SPINAL MONOAMINES BUT NOT OPIOIDS [J].
AIMONE, LD ;
JONES, SL ;
GEBHART, GF .
PAIN, 1987, 31 (01) :123-136
[2]  
AIMONE LD, 1986, J NEUROSCI, V6, P1803
[3]  
ALLGAIER C, 1988, ENDOCRINOLOGY, V122, P2699
[4]  
ALLGAIER C, 1986, AM J PHYSIOL, V251, pC833
[5]  
ASTONJONES G, 1991, PROG BRAIN RES, V88, P47
[6]   PROTEIN KINASE-C REGULATES IONIC CONDUCTANCE IN HIPPOCAMPAL PYRAMIDAL NEURONS - ELECTROPHYSIOLOGICAL EFFECTS OF PHORBOL ESTERS [J].
BARABAN, JM ;
SNYDER, SH ;
ALGER, BE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (08) :2538-2542
[7]  
BARTMANN P, 1989, NEUROSCIENCE, V29, P495
[8]   NEUROGENIC HYPERALGESIA - THE SEARCH FOR THE PRIMARY CUTANEOUS AFFERENT-FIBERS THAT CONTRIBUTE TO CAPSAICIN-INDUCED PAIN AND HYPERALGESIA [J].
BAUMANN, TK ;
SIMONE, DA ;
SHAIN, CN ;
LAMOTTE, RH .
JOURNAL OF NEUROPHYSIOLOGY, 1991, 66 (01) :212-227
[9]   DETERMINATION OF BRAIN INTERSTITIAL CONCENTRATIONS BY MICRODIALYSIS [J].
BENVENISTE, H ;
HANSEN, AJ ;
OTTOSEN, NS .
JOURNAL OF NEUROCHEMISTRY, 1989, 52 (06) :1741-1750
[10]   DESCENDING INHIBITORY INFLUENCES EXERTED BY BRAIN-STEM UPON ACTIVITIES OF DORSAL HORN LAMINA V-CELLS INDUCED BY INTRA-ARTERIAL INJECTION OF BRADYKININ INTO LIMBS [J].
BESSON, JM ;
GUILBAUD, G ;
LEBARS, D .
JOURNAL OF PHYSIOLOGY-LONDON, 1975, 248 (03) :725-&