Neural tube is partially dorsalized by overexpression of HrPax-37: The ascidian homologue of Pax-3 and Pax-7

被引:106
作者
Wada, H
Holland, PWH
Sato, S
Yamamoto, H
Satoh, N
机构
[1] KYOTO UNIV,GRAD SCH SCI,DEPT ZOOL,KYOTO 60601,JAPAN
[2] TOHOKU UNIV,GRAD SCH SCI,INST BIOL,SENDAI,MIYAGI 98077,JAPAN
基金
日本学术振兴会;
关键词
D O I
10.1006/dbio.1997.8626
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The origin and elaboration of the central nervous system played an important role in chordate and vertebrate history. All chordates possess a dorsal tubular central nervous system, but elaboration of dorsoventral and segmental pattern is far more pronounced in cephalochordates and vertebrates than in the more basal urochordates. Analysis of the urochordates, therefore, should allow deduction of the neural organization and neuronal patterning mechanisms that predated overt dorsoventral and segmental complexity. Here we report functional studies of the ascidian Pax gene (HrPax-37). The spatiotemporal expression pattern of HrPax-37 has suggested involvement in two distinct developmental processes: specification of dorsal cell fates of ectoderm during neurulation, and regional differentiation of the neural tube in later stages. Here we show that HrPax-37 is descendent from the precursor of the Pax-3 and Pax-7 genes implicated in specification of dorsal fate in the vertebrate neural tube. We also demonstrate that injection of HrPax-37 RNA into fertilized eggs causes ectopic expression of the dorsal neural marker tyrosinase gene in neurulae, confirming a regulatory role in dorsal patterning of the neural tube comparable to its vertebrate homologues. These results suggest that dorsal specification in the neural tube by Pax-3/7 subfamily genes was established in the ancestors of extant chordates during emergence of the dorsal tubular nervous system. (C) 1997 Academic Press.
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页码:240 / 252
页数:13
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