Nitrite reduction in Paracoccus sp is affected by a novel plasmid pYR1

被引:2
作者
Barak, Y
Yarmus, M
Shapira, R
van Rijn, J
机构
[1] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Dept Anim Sci, IL-76100 Rehovot, Israel
[2] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Ins Biochem Food Sci & Human Nutr, IL-76100 Rehovot, Israel
关键词
plasmid; nitrite reduction; Paracoccus;
D O I
10.1111/j.1574-6968.2002.tb11069.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two relatively low-copy plasmids of 9 and 16 kb were found to comprise the extrachromosomal DNA of a Paracoccus strain. Reduction of nitrate by plasmid-cured cells resulted in a significant intermediate nitrite accumulation as compared to wild-type cells. By examining nitrate reduction by transformants containing one of the two plasmids, it was found that nitrite accumulation was influenced by the 9.0-kb plasmid, designated as pYR1. Subcloning analysis showed that a 1.8-kb fragment of this plasmid affected nitrite accumulation. Sequence analysis of this fragment revealed the presence of five open reading frames. One of the six deduced proteins showed a strong homology to ABC transporters. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:111 / 116
页数:6
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