Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance technique

被引:74
作者
Arya, Sunil K.
Solanki, Pratima R.
Singh, Ravindra P.
Pandey, Manoj K.
Datta, Monika
Malhotra, Bansi D.
机构
[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Univ Delhi, Dept Chem, Delhi 110007, India
关键词
cholesterol; cholesterol oxidase (ChOx); surface plasmon resonance (SPR); octadecanethiol (ODT); 1-fluoro-2-nitro-4-azidobenzene (FNAB);
D O I
10.1016/j.talanta.2005.11.037
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Octadecanethiol (ODT) self-assembled monolayer (SAM) prepared onto gold-coated glass plate has been modified by using nitrene reaction of 1-fluoro-2-nitro-4-azidobenzene (FNAB) that further covalently binds to cholesterol oxidase (ChOx) via thermal reaction. FNAB acts as a bridge (cross-linker) between SAM and ChOx. The ChOx/FNAB/ODT/Au electrode thus fabricated has been characterized using contact angle (CA) measurements, UV-vis spectroscopy, electrochemical techniques and X-ray photoelectron spectroscopy (XPS) technique, respectively. This ChOx/FNAB/ODT/Au bioelectrode has been utilized for estimation of cholesterol in solution using surface plasmon resonance (SPR) technique. This SPR based cholesterol biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with lower detection limit of 50 mg/dl and shelf life of about 2 months when stored at 4 degrees C. (c) 2005 Elsevier B.V. All rights reserved.
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页码:918 / 926
页数:9
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