Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance technique

被引：74

作者：

Arya, Sunil K.

Solanki, Pratima R.

Singh, Ravindra P.

Pandey, Manoj K.

Datta, Monika

Malhotra, Bansi D.

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Univ Delhi, Dept Chem, Delhi 110007, India

来源：

TALANTA | 2006年 / 69卷 / 04期

关键词：

cholesterol; cholesterol oxidase (ChOx); surface plasmon resonance (SPR); octadecanethiol (ODT); 1-fluoro-2-nitro-4-azidobenzene (FNAB);

D O I：

10.1016/j.talanta.2005.11.037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Octadecanethiol (ODT) self-assembled monolayer (SAM) prepared onto gold-coated glass plate has been modified by using nitrene reaction of 1-fluoro-2-nitro-4-azidobenzene (FNAB) that further covalently binds to cholesterol oxidase (ChOx) via thermal reaction. FNAB acts as a bridge (cross-linker) between SAM and ChOx. The ChOx/FNAB/ODT/Au electrode thus fabricated has been characterized using contact angle (CA) measurements, UV-vis spectroscopy, electrochemical techniques and X-ray photoelectron spectroscopy (XPS) technique, respectively. This ChOx/FNAB/ODT/Au bioelectrode has been utilized for estimation of cholesterol in solution using surface plasmon resonance (SPR) technique. This SPR based cholesterol biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with lower detection limit of 50 mg/dl and shelf life of about 2 months when stored at 4 degrees C. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：918 / 926

页数：9

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