A mammal-specific Exon of WT1 is not required for development or fertility

被引：47

作者：

Natoli, TA

McDonald, A

Alberta, JA

Taglienti, ME

Housman, DE

Kreidberg, JA

机构：

[1] Childrens Hosp, Dept Med, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA

[3] MIT, Dept Biol, Ctr Canc Res, Cambridge, MA USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2002年 / 22卷 / 12期

关键词：

D O I：

10.1128/MCB.22.12.4433-4438.2002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The WT1 tumor suppressor gene is a zinc finger-containing transcription factor which is required for development of the kidney and gonads. A mammal-specific alternative splicing event within this gene results in the presence or absence of a 17-amino-acid sequence within the WT1 protein. To determine the function of this sequence in vivo, gene targeting was utilized to specifically eliminate the exon encoding this sequence in mice. Mice lacking WT1 exon 5 develop normally. Adult mice lacking this exon are viable and fertile, and females are capable of lactation.

引用

收藏

页码：4433 / 4438

页数：6

相关论文

共 29 条

[1] THE EXPRESSION OF THE WILMS-TUMOR GENE, WT1, IN THE DEVELOPING MAMMALIAN EMBRYO [J].

ARMSTRONG, JF ;

PRITCHARDJONES, K ;

BICKMORE, WA ;

HASTIE, ND ;

BARD, JBL .

MECHANISMS OF DEVELOPMENT, 1993, 40 (1-2) :85-97

[2] Donor splice-site mutations in WT1 are responsible for Frasier syndrome [J].

Barbaux, S ;

Niaudet, P ;

Gubler, MC ;

Grunfeld, JP ;

Jaubert, F ;

Kuttenn, F ;

Fekete, CN ;

SouleyreauTherville, N ;

Thibaud, E ;

Fellous, M ;

McElreavey, K .

NATURE GENETICS, 1997, 17 (04) :467-470

[3] ISOLATION, CHARACTERIZATION, AND EXPRESSION OF THE MURINE WILMS-TUMOR GENE (WT1) DURING KIDNEY DEVELOPMENT [J].

BUCKLER, AJ ;

PELLETIER, J ;

HABER, DA ;

GLASER, T ;

HOUSMAN, DE .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (03) :1707-1712

[4] ISOLATION AND CHARACTERIZATION OF A ZINC FINGER POLYPEPTIDE GENE AT THE HUMAN CHROMOSOME-11 WILMS TUMOR LOCUS [J].

CALL, KM ;

GLASER, T ;

ITO, CY ;

BUCKLER, AJ ;

PELLETIER, J ;

HABER, DA ;

ROSE, EA ;

KRAL, A ;

YEGER, H ;

LEWIS, WH ;

JONES, C ;

HOUSMAN, DE .

CELL, 1990, 60 (03) :509-520

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6]

Donovan MJ, 1999, DEV GENET, V24, P252, DOI 10.1002/(SICI)1520-6408(1999)24:3/4<252::AID-DVG8>3.3.CO

[7]

2-B

[8] HOMOZYGOUS DELETION IN WILMS-TUMORS OF A ZINC-FINGER GENE IDENTIFIED BY CHROMOSOME JUMPING [J].

GESSLER, M ;

POUSTKA, A ;

CAVENEE, W ;

NEVE, RL ;

ORKIN, SH ;

BRUNS, GAP .

NATURE, 1990, 343 (6260) :774-778

[9] ALTERNATIVE SPLICING AND GENOMIC STRUCTURE OF THE WILMS-TUMOR GENE-WT1 [J].

HABER, DA ;

SOHN, RL ;

BUCKLER, AJ ;

PELLETIER, J ;

CALL, KM ;

HOUSMAN, DE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9618-9622

[10] Two splice variants of the Wilms' tumor 1 gene have distinct functions during sex determination and nephron formation [J].

Hammes, A ;

Guo, JK ;

Lutsch, G ;

Leheste, JR ;

Landrock, D ;

Ziegler, U ;

Gubler, MC ;

Schedl, A .

CELL, 2001, 106 (03) :319-329