Measurement of hyperforin a constituent of St. John's wort in plasma by high-performance liquid chromatography

被引：19

作者：

Chi, JD ^{[1
]}

Franklin, M ^{[1
]}

机构：

[1] Univ Oxford, Warneford Hosp, Dept Psychiat, Psychopharmacol Res Unit, Oxford OX3 7JX, England

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1999年 / 735卷 / 02期

关键词：

hyperforin;

D O I：

10.1016/S0378-4347(99)00407-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hyperforin is a constituent of Hypericum perforatum extracts (St. John's wort, H. perforatum), which have antidepressant action. Hyperforin was extracted from plasma utilising a solid-phase extraction procedure. Chromatography was performed by isocratic reversed-phase high-performance Liquid chromatography with Wend-point detection. The calibration curve was linear over the range 0.15-3 mu g per ml of plasma. The sensitivity for hyperforin was 4.5 ng on-column. Mean inter- and intra-assay relative standard deviations over the range of the standard curve were less than 5%. The absolute recovery for hyperforin averaged 97.8%. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：285 / 288

页数：4

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