Genetic mapping of 262 loci derived from expressed sequences in a murine interspecific cross using single-strand conformational polymorphism analysis

被引：37

作者：

Brady, KP

Rowe, LB

Her, H

Stevens, TJ

Eppig, J

Sussman, DJ

Sikela, J

Beier, DR

机构：

[1] HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,DIV GENET,BOSTON,MA 02115

[2] JACKSON LAB,BAR HARBOR,ME 04605

[3] UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL,DENVER,CO 80262

[4] UNIV MARYLAND,SCH MED,DIV HUMAN GENET,BALTIMORE,MD 21201

来源：

GENOME RESEARCH | 1997年 / 7卷 / 11期

关键词：

D O I：

10.1101/gr.7.11.1085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.

引用

页码：1085 / 1093

页数：9

共 19 条

[1]

ADAMS MD, 1995, NATURE, V377, P3

[2] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].

ADAMS, MD ;

KELLEY, JM ;

GOCAYNE, JD ;

DUBNICK, M ;

POLYMEROPOULOS, MH ;

XIAO, H ;

MERRIL, CR ;

WU, A ;

OLDE, B ;

MORENO, RF ;

KERLAVAGE, AR ;

MCCOMBIE, WR ;

VENTER, JC .

SCIENCE, 1991, 252 (5013) :1651-1656

[3]

[Anonymous], 1996, GENETIC VARIANTS STR

[4] MAPPING GENES IN THE MOUSE USING SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS OF RECOMBINANT INBRED STRAINS AND INTERSPECIFIC CROSSES [J].

BEIER, DR ;

DUSHKIN, H ;

SUSSMAN, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9102-9106

[5] SINGLE-STRAND CONFORMATION POLYMORPHISM (SSCP) ANALYSIS AS A TOOL FOR GENETIC-MAPPING [J].

BEIER, DR .

MAMMALIAN GENOME, 1993, 4 (11) :627-631

[6] GENE-BASED SEQUENCE-TAGGED-SITES (STSS) AS THE BASIS FOR A HUMAN GENE MAP [J].

BERRY, R ;

STEVENS, TJ ;

WALTER, NAR ;

WILCOX, AS ;

RUBANO, T ;

HOPKINS, JA ;

WEBER, J ;

GOOLD, R ;

SOARES, MB ;

SIKELA, JM .

NATURE GENETICS, 1995, 10 (04) :415-423

[7] ESTABLISHING A HUMAN TRANSCRIPT MAP [J].

BOGUSKI, MS ;

SCHULER, GD .

NATURE GENETICS, 1995, 10 (04) :369-371

[8] A GENETIC-MAP OF THE MOUSE WITH 4,006 SIMPLE SEQUENCE LENGTH POLYMORPHISMS [J].

DIETRICH, WF ;

MILLER, JC ;

STEEN, RG ;

MERCHANT, M ;

DAMRON, D ;

NAHF, R ;

GROSS, A ;

JOYCE, DC ;

WESSEL, M ;

DREDGE, RD ;

MARQUIS, A ;

STEIN, LD ;

GOODMAN, N ;

PAGE, DC ;

LANDER, ES .

NATURE GENETICS, 1994, 7 (02) :220-245

[9] Generation and analysis of 280,000 human expressed sequence tags [J].

Hillier, L ;

Lennon, G ;

Becker, M ;

Bonaldo, MF ;

Chiapelli, B ;

Chissoe, S ;

Dietrich, N ;

DuBuque, T ;

Favello, A ;

Gish, W ;

Hawkins, M ;

Hultman, M ;

Kucaba, T ;

Lacy, M ;

Le, M ;

Le, N ;

Mardis, E ;

Moore, B ;

Morris, M ;

Parsons, J ;

Prange, C ;

Rifkin, L ;

Rohlfing, T ;

Schellenberg, K ;

Soares, MB ;

Tan, F ;

ThierryMeg, J ;

Trevaskis, E ;

Underwood, K ;

Wohldman, P ;

Waterston, R ;

Wilson, R ;

Marra, M .

GENOME RESEARCH, 1996, 6 (09) :807-828

[10] AN STS-BASED MAP OF THE HUMAN GENOME [J].

HUDSON, TJ ;

STEIN, LD ;

GERETY, SS ;

MA, JL ;

CASTLE, AB ;

SILVA, J ;

SLONIM, DK ;

BAPTISTA, R ;

KRUGLYAK, L ;

XU, SH ;

HU, XT ;

COLBERT, AME ;

ROSENBERG, C ;

REEVEDALY, MP ;

ROZEN, S ;

HUI, L ;

WU, XY ;

VESTERGAARD, C ;

WILSON, KM ;

BAE, JS ;

MAITRA, S ;

GANIATSAS, S ;

EVANS, CA ;

DEANGELIS, MM ;

INGALLS, KA ;

NAHF, RW ;

HORTON, LT ;

ANDERSON, MO ;

COLLYMORE, AJ ;

YE, WJ ;

KOUYOUMJIAN, V ;

ZEMSTEVA, IS ;

TAM, J ;

DEVINE, R ;

COURTNEY, DF ;

RENAUD, MT ;

NGUYEN, H ;

OCONNOR, TJ ;

FIZAMES, C ;

FAURE, S ;

GYAPAY, G ;

DIB, C ;

MORISSETTE, J ;

ORLIN, JB ;

BIRREN, BW ;

GOODMAN, N ;

WEISSENBACH, J ;

HAWKINS, TL ;

FOOTE, S ;

PAGE, DC .

SCIENCE, 1995, 270 (5244) :1945-1954

← 1 2 →