Picoeukaryote diversity in coastal waters of the Pacific Ocean

被引:147
作者
Worden, Alexandra Z. [1 ]
机构
[1] Univ Miami, Rosenstiel Sch Marine & Atmospher Sci, Div Marine Biol & Fisheries, Miami, FL 33134 USA
关键词
picoeukaryotes; picophytoplankton; picoplankton; diversity; Micromonas pusilla; prasinophytes; 18S rRNA; small subunit;
D O I
10.3354/ame043165
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The extraordinary molecular phylogenetic diversity of picoeukaryotes and their contributions to marine processes have been highlighted recently. Herein, picoeukaryotic diversity in the coastal Pacific Ocean is characterized for the first time, Close to full length small subunit ribosomal RNA (ssu rRNA) gene amplicons were cloned and sequenced from size fractionated samples (< 2 mu m) taken between September 2000 and October 2001. Sequences belonging to the order Mamiellales were abundant, with Micromonas pusilla-like sequences in all 5 libraries (4 operational taxonomic units, OTUs, at >= 99% sequence similarity), Ostreococcus in 4 (1 OTU) and Bathycoccus in 2 (1 OTU). Phylogenetic reconstruction showed distinct Micromonas clades at this site (although not for other Mamiellales), supporting the idea that the designation M pusilla harbors cryptic species. In combination with picophytoplankton dynamics previously reported for the same period, the data indicates that picoplanktonic primary production at this site is likely to be dominated by prasinophytes. Furthermore, a Micromonas-clade with no cultured representatives was identified bearing highest identity to Sargasso Sea shotgun clone sequences. Comparison of the Pacific sequences with the shotgun clones showed Ostreococcus and Micromonas at 1 Sargasso site with elevated chlorophyll (chl) levels, but not at other Sargasso sites. Other Pacific clones were primarily Novel Alveolate Group II, which were highly diverse based on OTU analyses. Novel Alveolate Group 1, Ciliophora, Cercozoa-like and stramenopile sequences were also retrieved. Although picoeukaryotic diversity has been characterized in only 1 other Pacific Ocean sample (equatorial Pacific), most stramenopile and alveolate sequences corresponded to previously identified phylogenetic clades from studies conducted in other oceans and for which no cultured representatives exist.
引用
收藏
页码:165 / 175
页数:11
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共 46 条
  • [1] A comparison of HPLC pigment signatures and electron microscopic observations for oligotrophic waters of the North Atlantic and Pacific Oceans
    Andersen, RA
    Bidigare, RR
    Keller, MD
    Latasa, M
    [J]. DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 1996, 43 (2-3) : 517 - 537
  • [2] Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis
    Castresana, J
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 2000, 17 (04) : 540 - 552
  • [3] Phylogeny and classification of phylum Cercozoa (Protozoa)
    Cavalier-Smith, T
    Chao, EEY
    [J]. PROTIST, 2003, 154 (3-4) : 341 - 358
  • [4] The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy
    Cole, JR
    Chai, B
    Marsh, TL
    Farris, RJ
    Wang, Q
    Kulam, SA
    Chandra, S
    McGarrell, DM
    Schmidt, TM
    Garrity, GM
    Tiedje, JM
    [J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (01) : 442 - 443
  • [5] Protistan diversity estimates based on 18S rDNA from seawater incubations in the western North Atlantic
    Countway, PD
    Gast, RJ
    Savai, P
    Caron, DA
    [J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2005, 52 (02) : 95 - 106
  • [6] Abundance and distribution of Ostreococcus sp in the San Pedro Channel, California, as revealed by quantitative PCR
    Countway, PD
    Caron, DA
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (04) : 2496 - 2506
  • [7] DERELLE E, IN PRESS P NATL ACAD
  • [8] Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing
    Díez, B
    Pedrós-Alió, C
    Massana, R
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) : 2932 - 2941
  • [9] Florenciella parvuld gen. et sp nov (Dictyochophyceae, Heterokontophyta), a small flagellate isolated from the English Channel
    Eikrem, W
    Romari, K
    Latasa, M
    Le Gall, F
    Throndsen, J
    Vaulot, D
    [J]. PHYCOLOGIA, 2004, 43 (06) : 658 - 668
  • [10] Variations in bacterial community structure during a dinoflagellate bloom analyzed by DGGE and 16S rDNA sequencing
    Fandino, LB
    Riemann, L
    Steward, GF
    Long, RA
    Azam, F
    [J]. AQUATIC MICROBIAL ECOLOGY, 2001, 23 (02) : 119 - 130