Mannanase Man26A from Cellulomonas fimi has a mannan-binding module

被引：19

作者：

Stoll, D

Boraston, A

Stålbrand, H

McLean, BW

Kilburn, DG

Warren, RAJ

机构：

[1] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z3, Canada

[2] Univ British Columbia, Dept Biotechnol Lab, Vancouver, BC V6T 1Z3, Canada

[3] Univ British Columbia, Prot Engn Network, Ctr Excellence, Vancouver, BC V6T 1Z3, Canada

来源：

FEMS MICROBIOLOGY LETTERS | 2000年 / 183卷 / 02期

关键词：

Cellulomonas fimi; mannanase; mannan-binding module; affinity gel electrophoresis;

D O I：

10.1111/j.1574-6968.2000.tb08969.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble mannans. Man26Abm does not bind to cellulose, chitin or xylan. The K-d for binding of Man26Abm to locust bean gum (LBG) is similar to 0.2 mu M Man26A is the first mannanase reported to contain a mannan-binding module. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science 8.V. All rights reserved.

引用

页码：265 / 269

页数：5

共 21 条

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