Analysis of phosphoinositides in protein trafficking

被引：36

作者：

Hama, H ^{[1
]}

Takemoto, JY ^{[1
]}

DeWald, DB ^{[1
]}

机构：

[1] Utah State Univ, Dept Biol, Logan, UT 84322 USA

来源：

METHODS | 2000年 / 20卷 / 04期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1006/meth.2000.0959

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphoinositides are key regulators of vesicle-mediated protein trafficking Their roles include recruiting vesicle coat and effector proteins to the site of budding and promoting vesicle fusion. The intracellular levels of phosphoinositides and their localization to intracellular membranes are critical to their functions. An analytical procedure was developed that optimizes the recovery of radiolabeled cellular phosphoinositides. Quantitative analyses of yeast cellular phosphoinositides indicated that this approach is useful for examining the intracellular membrane phosphoinositide compositions related to trafficking phenomena. The approach will also enable investigators to determine whole-plant phosphoinositide compositions that have been difficult to achieve in the past. These analytical advances should be generally applicable to studies of phosphoinositide dynamics related to membrane trafficking in yeast, plant, and animal cells. (C) 2000 Academic Press.

引用

页码：465 / 473

页数：9

共 39 条

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