ANTHOCYANIN1 of petunia controls pigment synthesis, vacuolar pH, and seed coat development by genetically distinct mechanisms

被引:188
作者
Spelt, C [1 ]
Quattrocchio, F [1 ]
Mol, J [1 ]
Koes, R [1 ]
机构
[1] Vrije Univ Amsterdam, Dept Dev Genet, Inst Mol Biol Sci, NL-1081 HV Amsterdam, Netherlands
关键词
D O I
10.1105/tpc.003772
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ANTHOCYANIN1 (AN1) of petunia is a transcription factor of the basic helix-loop-helix (bHLH) family that is required for the synthesis of anthocyanin pigments. Here, we show that AN1 controls additional aspects of cell differentiation: the acidification of vacuoles in petal cells, and the size and morphology of cells in the seed coat epidermis. We identified an1 alleles, formerly known as ph6, that sustain anthocyanin synthesis but not vacuolar acidification and seed coat morphogenesis. These alleles express truncated proteins lacking the C-terminal half of AN1, including the bHLH domain, at an similar to30-fold higher level than wild-type AN1. An allelic series in which one, two, or three amino acids were inserted into the bHLH domain indicated that this domain is required for both anthocyanin synthesis and vacuolar acidification. These findings show that AN1 controls more aspects of epidermal cell differentiation than previously thought through partially separable domains.
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收藏
页码:2121 / 2135
页数:15
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共 48 条
  • [1] Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases
    Alfenito, MR
    Souer, E
    Goodman, CD
    Buell, R
    Mol, J
    Koes, R
    Walbot, V
    [J]. PLANT CELL, 1998, 10 (07) : 1135 - 1149
  • [2] REGULATION OF GENE ACTION IN PETUNIA-HYBRIDA - UNSTABLE ALLELES OF A GENE FOR FLOWER COLOR
    BIANCHI, F
    CORNELISSEN, PTJ
    GERATS, AGM
    HOGERVORST, JMW
    [J]. THEORETICAL AND APPLIED GENETICS, 1978, 53 (04) : 157 - 167
  • [3] Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis
    Borevitz, JO
    Xia, YJ
    Blount, J
    Dixon, RA
    Lamb, C
    [J]. PLANT CELL, 2000, 12 (12) : 2383 - 2393
  • [4] Expression profiling of the maize flavonoid pathway genes controlled by estradiol-inducible transcription factors CRC and P
    Bruce, W
    Folkerts, O
    Garnaat, C
    Crasta, O
    Roth, B
    Bowen, B
    [J]. PLANT CELL, 2000, 12 (01) : 65 - 79
  • [5] CHUCK G, 1993, PLANT CELL, V5, P371, DOI 10.1105/tpc.5.4.371
  • [6] Downregulation of ovule-specific MADS box genes from petunia results in maternally controlled defects in seed development
    Colombo, L
    Franken, J
    VanderKrol, AR
    Wittich, PE
    Dons, HJM
    Angenent, GC
    [J]. PLANT CELL, 1997, 9 (05) : 703 - 715
  • [7] Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis
    Debeaujon, I
    Léon-Kloosterziel, KM
    Koornneef, M
    [J]. PLANT PHYSIOLOGY, 2000, 122 (02) : 403 - 413
  • [8] The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium
    Debeaujon, I
    Peeters, AJM
    Léon-Kloosterziel, KM
    Koornneef, M
    [J]. PLANT CELL, 2001, 13 (04) : 853 - 871
  • [9] The an11 locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals
    deVetten, N
    Quattrocchio, F
    Mol, J
    Koes, R
    [J]. GENES & DEVELOPMENT, 1997, 11 (11) : 1422 - 1434
  • [10] GENES AFFECTING FLOWER COLOR AND PH OF FLOWER LIMB HOMOGENATES IN PETUNIA-HYBRIDA
    DEVLAMING, P
    SCHRAM, AW
    WIERING, H
    [J]. THEORETICAL AND APPLIED GENETICS, 1983, 66 (3-4) : 271 - 278