Integrated physical, genetic, and genic map covering 3 Mb around the human NGF gene (NGFB) at 1p13

被引：7

作者：

Carrier, A

Rosier, MF

Guillemot, F

Goguel, AF

Pulcini, F

Bernheim, A

Auffray, C

Devignes, MD

机构：

[1] CNRS UPR 420, F-94801 VILLEJUIF, FRANCE

[2] INST GUSTAVE ROUSSY, LAB CYTOGENET & GENET ONCOL, CNRS URA 1967, F-94805 VILLEJUIF, FRANCE

[3] GENETHON, GENEXPRESS, F-91002 EVRY, FRANCE

来源：

GENOMICS | 1996年 / 31卷 / 01期

关键词：

D O I：

10.1006/geno.1996.0012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have characterized 11 overlapping yeast artificial chromosomes (YACs) in the lp13 region, 8 of them containing the human nerve growth factor (NGF) gene (HGMW-approved symbol NGFB). Sequence-tagged sites (STSs) corresponding to YAC extremities have been designed and used for chromosome assignment on a panel of monochromosomic somatic cell hybrids to check for YAC chimerism, in parallel with analyses by fluorescence in situ hybridization. Determination of end STS content and restriction mapping of the YACs led to the construction of a 3-Mb YAC contig. Four microsatellite markers from the Genethon collection and seven genes known to map to the lp13 region have been ordered on the contig around the NGF gene. A new gene transcript from the Genexpress catalog has been localized on the contig. This work provides an integrated physical, genetic, and genic map of this chromosome 1 region. It constitutes a basis for determining the structure of the NGF gene and for further characterizing its genic environment. (C) 1996 Academic Press, Inc.

引用

页码：80 / 89

页数：10

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