Characterization of an exocellular β-glucosidase from Debaryomyces pseudopolymorphus

被引：42

作者：

Villena, Maria Arevalo ^{[1
]}

Iranzo, Juan F. Ubeda

Gundllapalli, Sarath B.

Otero, Ricardo R. Cordero

Perez, Ana I. Briones

机构：

[1] Univ Castilla La Mancha, Dept Quim Analit, Ciudad Real 13071, Spain

[2] Univ Stellenbosch, Dept Viticulture & Oenol, Inst Wine Biotechnol, ZA-7600 Stellenbosch, South Africa

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

beta-glucosidase; Debaryomyces pseudopolymorphus; exocellular; glucose resistance; wine aroma;

D O I：

10.1016/j.enzmictec.2005.10.018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

When grown in complex media containing 20 g of cellobiose per litre, Debaryomyces pseudopolymorphus secreted a P-glucosidase. The synthesis of this enzyme was repressed by glucose. Most of the enzyme was concentrated in the supernatant, with only 10% of the total activity being cell associated. This beta-glucosidase (designated Dp-beta gl) was purified and shown to be a monomer with a native molecular mass of approximately 100,000 Da. It demonstrated optimal activity at a pH of 4 and, in the short term (no more than 2 h), at a temperature of 40 degrees C. Temperature-stability analysis revealed that the enzyme was labile at 50 degrees C and above. It had a strong affinity for cellobiose and maltose, and degraded larninarin. It was inhibited by Ca++, Zn++, Mg++ and acetic acid, but apparently not by glucose and ethanol. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：229 / 234

页数：6

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