Cholesterol-linked fluorescent molecular beacons with enhanced cell permeability

被引：26

作者：

Seo, Young Jun

Jeong, Hyun Seok

Bang, Eun-Kyoung

Hwang, Gil Tae

Jung, Jong Ha

Jang, Sung Key

Kim, Byeang Hyean

机构：

[1] Pohang Univ Sci & Technol, Dept Chem, Natl Res Lab, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol, Dept Life Sci, Div Mol & Life Sci, Pohang 790784, South Korea

来源：

BIOCONJUGATE CHEMISTRY | 2006年 / 17卷 / 05期

关键词：

D O I：

10.1021/bc060078q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We appended pyrene units covalently onto adenosine (forming A(P) units) and then incorporated them into oligonucleotides such that they were positioned in complementary locations in opposite strands in the middle positions of hairpin stems. System 1 (A(P)A(P)) behaves as an effective molecular beacon (MB) that changes color from green to blue upon duplex formation. In addition, we attached a cholesterol unit to a free terminus of one of these hairpins; this approach enhanced the cellular delivery of the modified MB relative to those encountered when using conventional transfection methods. These structurally simple cholesterol-based MB systems, which can be synthesized very efficiently, have good potential for opening up new and exciting opportunities in the field of in vivo biosensors.

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收藏

页码：1151 / 1155

页数：5

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