Analysis of chaperonin-containing TCP-1 subunits in the human keratinocyte two-dimensional protein database: Further characterisation of antibodies to individual subunits

被引:14
作者
Hynes, G
Celis, JE
Lewis, VA
Carne, A
U, S
Lauridsen, JB
Willison, KR
机构
[1] INST CANC RES,CHESTER BEATTY LABS,CRC,CTR CELL & MOL BIOL,LONDON SW3 6JB,ENGLAND
[2] AARHUS UNIV,DEPT BIOCHEM MED,AARHUS,DENMARK
[3] AARHUS UNIV,DANISH CTR HUMAN GENOME RES,AARHUS,DENMARK
关键词
chaperonin-containing TCP-1; human keratinocyte two-dimensional protein database; antibody characterisation; epitope mapping;
D O I
10.1002/elps.1150171109
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The chaperonin-containing TCP-1 (CCT), found in the eukaryotic cytosol, is currently the focus of extensive research. CCT consists of at least eight different subunit types encoded by independent but related genes, and a set of antibodies that recognise individual subunits has proved useful in the characterisation and functional analysis of CCT. These antibodies were used to identify subunits of CCT in the human keratinocyte two-dimensional protein database. Accurate values for the pi and molecular mass of human CCT subunits were determined from the database, and biological data was obtained regarding changes in subunit levels in response to extracellular agents and growth conditions. The second part of the study describes the characterisation of seven monoclonal antibodies raised against mouse TCP-1, also known as CCT alpha, using a combination of epitope mapping and immunoblot analysis of protein extracts from different species and tissue types. Some antibodies were not monospecific for TCP-1, and a number of epitope-related proteins were identified.
引用
收藏
页码:1720 / 1727
页数:8
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