Licania michauxii Prance root extract induces hsp 70 mRNA and necrotic cell death in cultured human hepatoma and colon carcinoma cell lines

被引：27

作者：

Badisa, RB

Chaudhuri, SK

Pilarinou, E

Rutkoski, NJ

Hare, J

Levenson, CW ^{[1
]}

机构：

[1] Florida State Univ, Dept Nutr Food & Exercise Sci, Biomed Res Facil, Tallahassee, FL 32308 USA

[2] Walker Canc Res Inst, Tallahassee, FL 32308 USA

[3] Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USA

来源：

CANCER LETTERS | 2000年 / 149卷 / 1-2期

关键词：

apoptosis; p53; hsp; 70; necrosis; cytotoxicity; HepG2; Caco-2;

D O I：

10.1016/S0304-3835(99)00347-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

An extract of Licania michauxii Prance root was found to be cytotoxic to cultured human hepatoma (HepG2) and colon carcinoma (Caco-2) cells. Morphological and nuclear characteristics of treated cells were consistent with necrotic death. Increases in the chaperone protein hsp 70 and hsp 70 mRNA were dose dependent reaching peak mRNA levels (40-fold above control) at 6 h. Increases in nuclear localization of hsp 70 was also observed with treatment. Heat treatment of cells for 45 min to induce hsp 70 prior to treatment with the extract provided transient protection from the necrotic response. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

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页码：61 / 68

页数：8

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