Diagnosis of group A coxsackieviral infection using polymerase chain reaction

被引:11
作者
Hosoya, M
Ishiko, H
Shimada, Y
Honzumi, K
Suzuki, S
Kato, K
Suzuki, H
机构
[1] Fukushima Med Univ, Sch Med, Dept Pediat, Fukushima 9601295, Japan
[2] Fukushima Inst Publ Hlth & Environm Sci, Fukushima 9608560, Japan
[3] Mitsubishi Kagaku Bioclin Labs Inc, Infect Dis Lab, Tokyo 1748555, Japan
关键词
D O I
10.1136/adc.87.4.316
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Aims: To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR). Methods: Throat swabs were collected from 246 children from June to August 1997 and 1998. Results: Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Enteroviral genomes were detected in 54/178 samples from which no virus was isolated. Of 41 enteroviral genotypes identified by sequence analysis of PCR products, 38 were group A coxsackieviruses, which are usually difficult to isolate using tissue culture. Conclusion: Results indicate that viral detection and identification based on PCR is useful in the diagnosis of group A coxsackieviral infection.
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收藏
页码:316 / 319
页数:4
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